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stata v 15 1  (STATA Corporation)


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    STATA Corporation stata v 15 1
    Stata V 15 1, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 38892 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v+15/pmc13052182-89-4-4?v=STATA+Corporation
    Average 99 stars, based on 38892 article reviews
    stata v 15 1 - by Bioz Stars, 2026-07
    99/100 stars

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    UCHL1 is positively associated with TMZ resistance in glioblastoma cells. (A) Cell viability curves for the U87MG, T98G, and U251 cell lines following TMZ treatment were evaluated using the CCK-8 assay. The IC50 values were determined through nonlinear regression analysis (curve fitting), with three replicates ( n = 3). (B) The IC50 values for TMZ-treated U251 cells were assessed after exposure to 100 nM MG132 (a proteasome inhibitor) or 5 μM CQ (an autophagy/lysosome inhibitor) ( n = 3). (C) Differential genes from the datasets GSE193957 , GSE211272 , GSE229600 , and GSE113510 were intersected with members of the ubiquitin proteasome family. Western blotting analysis (D) and RT-qPCR (E) were conducted to assess UCHL1 levels in the U87MG, T98G, and U251 cell lines ( n = 3). * p < 0.05, *** p < 0.001.

    Journal: Translational Oncology

    Article Title: UCHL1 promotes temozolomide resistance in glioblastoma by inhibiting the ubiquitination-mediated degradation of keratin 8

    doi: 10.1016/j.tranon.2026.102728

    Figure Lengend Snippet: UCHL1 is positively associated with TMZ resistance in glioblastoma cells. (A) Cell viability curves for the U87MG, T98G, and U251 cell lines following TMZ treatment were evaluated using the CCK-8 assay. The IC50 values were determined through nonlinear regression analysis (curve fitting), with three replicates ( n = 3). (B) The IC50 values for TMZ-treated U251 cells were assessed after exposure to 100 nM MG132 (a proteasome inhibitor) or 5 μM CQ (an autophagy/lysosome inhibitor) ( n = 3). (C) Differential genes from the datasets GSE193957 , GSE211272 , GSE229600 , and GSE113510 were intersected with members of the ubiquitin proteasome family. Western blotting analysis (D) and RT-qPCR (E) were conducted to assess UCHL1 levels in the U87MG, T98G, and U251 cell lines ( n = 3). * p < 0.05, *** p < 0.001.

    Article Snippet: After 48 h of incubation, 10% (v/v) Cell counting kit-8 (CCK-8) reagent (Dojindo, Kumamoto, Japan) was added to each well.

    Techniques: CCK-8 Assay, Ubiquitin Proteomics, Western Blot, Quantitative RT-PCR